Composite

Part:BBa_K676777:Design

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)


GyrA cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis is carried out to remove the restriction site.


Source

Gyr A gene is obtained from E.coli genome.

References